| Host: |
Goat/Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This ISG15 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Purification: |
Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Determination Method: |
Colormetric |
| Gene Symbol: |
ISG15 |
| Gene ID: |
9636 |
| Uniprot ID: |
ISG15_HUMAN |
| Immunogen: |
Capture immunogen: Recombinant Human ISG15 protien.Detection Immunogen: Recombinant Human ISG15 protien. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | S-nitrosylation decreases its dimerization, thereby increasing the availability as well as the solubility of monomeric ISG15 for its conjugation to cellular proteins. Induced as an inactive, precursor protein that is cleaved by specific proteases to expose the C-terminal diglycine (LRLRGG) motif. This motif is essential not only for its conjugation to substrates but also for its recognition by the relevant processing proteases. |
| Function | Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Isgylation of the viral sensor IFIH1/MDA5 promotes IFIH1/MDA5 oligomerization and triggers activation of innate immunity against a range of viruses, including coronaviruses, flaviviruses and picornaviruses. Can also isgylate: EIF2AK2/PKR which results in its activation, RIGI which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade thereby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10.the interaction is mediated by ITGAL. |
| Protein Name | Ubiquitin-Like Protein Isg15Interferon-Induced 15 Kda ProteinInterferon-Induced 17 Kda ProteinIp17Ubiquitin Cross-Reactive ProteinHucrp |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168276Reactome: R-HSA-168928Reactome: R-HSA-5656169Reactome: R-HSA-909733Reactome: R-HSA-936440Reactome: R-HSA-9705671Reactome: R-HSA-9833110Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmSecretedExists In Three Distinct States: Free Within The CellReleased Into The Extracellular SpaceOr Conjugated To Target Proteins |
| Alternative Antibody Pair Names | Ubiquitin-Like Protein Isg15 antibody pairInterferon-Induced 15 Kda Protein antibody pairInterferon-Induced 17 Kda Protein antibody pairIp17 antibody pairUbiquitin Cross-Reactive Protein antibody pairHucrp antibody pairISG15 antibody pairG1P2 antibody pairUCRP antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ISG15 Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] ISG15 Rabbit polyclonal antibody (STJ11104641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177285/368924/STJ11104641_1__76093.1713134403.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ISG15 Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] ISG15 Rabbit polyclonal antibody (STJ11104641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")