• Human IL10 (Interleukin-10) CLIA Kit (STJC0000093)

Human IL10 (Interleukin-10) CLIA Kit (STJC0000093)

SKU:
STJC0000093

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human IL10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL10
Gene ID: 3586
Uniprot ID: IL10_HUMAN
Specificity: This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Function Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Protein Name Interleukin-10
Il-10
Cytokine Synthesis Inhibitory Factor
Csif
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8950505
Reactome: R-HSA-9662834
Reactome: R-HSA-9664323
Cellular Localisation Secreted
Alternative CLIA Names Interleukin-10 CLIA kit
Il-10 CLIA kit
Cytokine Synthesis Inhibitory Factor CLIA kit
Csif CLIA kit
IL10 CLIA kit
Specificity This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 98
EDTA plasma(n=8) 90-101 95
Cell culture media(n=8) 86-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 24.45 46.94 167.57 22.04 49.29 171.49
Standard deviation 3.13 5.51 16.12 2.52 5.52 13.31
CV (%) 12.80 11.74 9.62 11.43 11.20 7.76
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance