Human IL-6R (Interleukin-6 Receptor Subunit Alpha) Antibody Pair (STJAP00259)

SPECIFICATIONS
ClonalityPolyclonal/Polyclonal
HostGoat/Goat
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human IL-6R protein.
Detection Immunogen: Recombinant Human IL-6R protein.
STJAP00259-50
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General Information

Short DescriptionThis IL-6R antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA.
ApplicationsELISA
HostGoat/Goat
ReactivityHuman
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

ClonalityPolyclonal/Polyclonal
ConjugationUnconjugated/Biotin
Concentration0.5 mg/mL
PurificationCapture Purification: Antigen Affinity.
Detection Purification: Antigen Affinity.
FormulationCapture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Storage InstructionStore at-20°C for the long term, and avoid freeze-thaw cycles.

Target Information

Gene SymbolIL6R
Gene ID3570
Uniprot IDIL6RA_HUMAN
ImmunogenCapture immunogen: Recombinant Human IL-6R protein.
Detection Immunogen: Recombinant Human IL-6R protein.

Additional Info

Post Translational Modifications A short soluble form is released from the membrane by proteolysis. The sIL6R is formed mostly by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, but is also directly secreted from the cells after alternative mRNA splicing. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis.
Function Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). Isoform 1: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Isoform 2: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis.
Protein Name Interleukin-6 Receptor Subunit Alpha
Il-6 Receptor Subunit Alpha
Il-6r Subunit Alpha
Il-6r-Alpha
Il-6ra
Il-6r 1
Membrane Glycoprotein 80
Gp80
Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha
Sil6r
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-6785807
Reactome: R-HSA-9616222
Reactome: R-HSA-9679191
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Soluble Interleukin-6 Receptor Subunit Alpha: Secreted
Alternative Antibody Pair Names Interleukin-6 Receptor Subunit Alpha antibody pair
Il-6 Receptor Subunit Alpha antibody pair
Il-6r Subunit Alpha antibody pair
Il-6r-Alpha antibody pair
Il-6ra antibody pair
Il-6r 1 antibody pair
Membrane Glycoprotein 80 antibody pair
Gp80 antibody pair
Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha antibody pair
Sil6r antibody pair
IL6R antibody pair

Information sourced from Uniprot.org

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