| Host: |
CHO cells |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human IL-35-protein was developed from cho cells. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution pH 7.4. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was measured using Immobilized Human IL-35 ability to bind biotinylated Human IL6RB in ELISA. NA |
| Gene Symbol: |
EBI3 |
| Gene ID: |
10148 |
| Uniprot ID: |
IL27B_HUMAN |
| Immunogen Region: |
Mature chain |
| Immunogen: |
DNA sequence encoding Human IL27B fused toDNA sequence encoding mature chain of Human IL12 p35 via linker chain and Human IgG1 Fc C-terminal tag was expressed in CHO cells. NA |
| Function | Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. |
| Protein Name | Interleukin-27 Subunit BetaIl-27 Subunit BetaIl-27bEpstein-Barr Virus-Induced Gene 3 ProteinEbv-Induced Gene 3 Protein |
| Database Links | Reactome: R-HSA-8984722Reactome: R-HSA-9020956 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-27 Subunit Beta proteinIl-27 Subunit Beta proteinIl-27b proteinEpstein-Barr Virus-Induced Gene 3 Protein proteinEbv-Induced Gene 3 Protein proteinEBI3 proteinIL27B protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
