| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IL16 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant human CD4 with a linear range of 0.2-1 Mu g/mL. |
| Gene Symbol: | IL16 |
| Gene ID: | 3603 |
| Uniprot ID: | IL16_HUMAN |
| Immunogen Region: | Pro2-Ser130 |
| Immunogen: | Recombinant Human IL-16 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro2-Ser130) of human IL-16 (Accession #NP_757366.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | PDLNSSTDSAASASAASDVS VESTAEATVCTVTLEKMSAG LGFSLEGGKGSLHGDKPLTI NRIFKGAASEQSETVQPGDE ILQLGGTAMQGLTRFEAWNI IKALPDGPVTIVIRRKSLQS KETTAAGDS |
| Tissue Specificity | Isoform 3: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation. |
| Post Translational Modifications | Isoform 3: Synthesized as a chemo-attractant inactive precursor in hemopoietic tissues, and proteolytically cleaved by caspase-3 to yield IL-16. |
| Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1: May act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. |
| Protein Name | Pro-Interleukin-16 Cleaved Into - Interleukin-16 Il-16 Lymphocyte Chemoattractant Factor Lcf |
| Database Links | Reactome: R-HSA-449836 |
| Cellular Localisation | Interleukin-16: Secreted Isoform 1: Cytoplasm Isoform 3: Cytoplasm Nucleus |
| Alternative Protein Names | Pro-Interleukin-16 Cleaved Into - Interleukin-16 protein Il-16 protein Lymphocyte Chemoattractant Factor protein Lcf protein IL16 protein |
Information sourced from Uniprot.org