| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human IGFBP7 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
IGFBP7 |
| Gene ID: |
3490 |
| Uniprot ID: |
IBP7_HUMAN |
| Specificity: |
This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. |
| Function | Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 7Ibp-7Igf-Binding Protein 7Igfbp-7Igfbp-Rp1Mac25 ProteinPgi2-Stimulating FactorProstacyclin-Stimulating FactorTumor-Derived Adhesion FactorTaf |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Insulin-Like Growth Factor-Binding Protein 7 CLIA kitIbp-7 CLIA kitIgf-Binding Protein 7 CLIA kitIgfbp-7 CLIA kitIgfbp-Rp1 CLIA kitMac25 Protein CLIA kitPgi2-Stimulating Factor CLIA kitProstacyclin-Stimulating Factor CLIA kitTumor-Derived Adhesion Factor CLIA kitTaf CLIA kitIGFBP7 CLIA kitMAC25 CLIA kitPSF CLIA kit |
| Specificity | This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-105 | 97 |
| EDTA plasma(n=8) | 85-97 | 90 |
| Cell culture media(n=8) | 95-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.55 | 1.58 | 4.07 | 0.58 | 1.60 | 4.41 |
| Standard deviation | 0.07 | 0.19 | 0.38 | 0.07 | 0.15 | 0.34 |
| CV (%) | 12.73 | 12.03 | 9.34 | 12.07 | 9.38 | 7.71 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
