| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.01 EU/Mu g |
| Immunogen Region: | Arg99-Lys384 |
| Immunogen: | Recombinant Human IgD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg99-Lys384) of Human IgD (Accession #P01880-1) fused with a His tag at the C-terminus. |
| Immunogen Sequence: | QQTRQEADRGCETLVVQHGH CSYTFLLPKSEPCPPGPEVS RDSNTLQRESLANPLHLGKL PTQQVKQLEQALQNNTQWLK KLERAIKTILRSKLEQVQQQ MAQNQTAPMLELGTSLLNQT TAQIRKLTDMEAQLLNQTSR MDAQMPETFLSTNKLENQLL LQRQKLQQLQGQNSALEKRL QALETKQQEELASILSKKAK LLNTLSRQSAALTNIERGLR GVRHNSSLLQDQQHSLRQL |
| Background | IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD. |
Information sourced from Uniprot.org
