| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-3 coated plates (12.5 Mu g/mL, 100 Mu L/well) , approximately >50% cells will adhere specifically after 60 minutes at 37 °C. |
| Gene Symbol: | ICAM3 |
| Gene ID: | 3385 |
| Uniprot ID: | ICAM3_HUMAN |
| Immunogen: | Recombinant Human ICAM-3/CD50 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln30-His485) of human CD50 (Accession #NP_002153.2) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | QEFLLRVEPQNPVLSAGGSL FVNCSTDCPSSEKIALETSL SKELVASGMGWAAFNLSNVT GNSRILCSVYCNGSQITGSS NITVYRLPERVELAPLPPWQ PVGQNFTLRCQVEDGSPRTS LTVVLLRWEEELSRQPAVEE PAEVTATVLASRDDHGAPFS CRTELDMQPQGLGLFVNTSA PRQLRTFVLPVTPPRLVAPR FLEVETSWPVDCTLDGLFPA SEAQVYLALGDQMLNATVM |
| Tissue Specificity | Leukocytes. |
| Post Translational Modifications | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated.glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. |
| Protein Name | Intercellular Adhesion Molecule 3Icam-3Cdw50Icam-RCd Antigen Cd50 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-5621575 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Intercellular Adhesion Molecule 3 proteinIcam-3 proteinCdw50 proteinIcam-R proteinCd Antigen Cd50 proteinICAM3 protein |
Information sourced from Uniprot.org