| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This human HGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | HGF |
| Gene ID: | 3082 |
| Uniprot ID: | HGF_HUMAN |
| Specificity: | This kit recognizes Human HGF in samples. No significant cross-reactivity or interference between Human HGF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form. |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6806942Reactome: R-HSA-6807004Reactome: R-HSA-6811558Reactome: R-HSA-8851805Reactome: R-HSA-8851907Reactome: R-HSA-8865999Reactome: R-HSA-8874081Reactome: R-HSA-8875513Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-8875791Reactome: R-HSA-9734091 |
| Alternative CLIA Names | Hepatocyte Growth Factor CLIA kitHepatopoietin-A CLIA kitScatter Factor CLIA kitSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain CLIA kitHGF CLIA kitHPTA CLIA kit |
| Specificity | This kit recognizes Human HGF in samples. No significant cross-reactivity or interference between Human HGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-106 | 100 |
| EDTA plasma(n=8) | 86-99 | 92 |
| Cell culture media(n=8) | 91-108 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.98 | 235.21 | 773.47 | 101.17 | 229.84 | 801.84 |
| Standard deviation | 10.70 | 25.87 | 84.85 | 10.91 | 20.59 | 60.78 |
| CV (%) | 10.19 | 11.00 | 10.97 | 10.78 | 8.96 | 7.58 |