| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human HCK-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | HCK |
| Gene ID: | 3055 |
| Uniprot ID: | HCK_HUMAN |
| Immunogen Region: | Met22-Pro526 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases. Ubiquitinated by CBL, leading to its degradation via the proteasome. Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2. |
| Function | Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. |
| Protein Name | Tyrosine-Protein Kinase HckHematopoietic Cell KinaseHemopoietic Cell KinaseP59-Hck/P60-HckP59hckP61hck |
| Database Links | Reactome: R-HSA-164944Reactome: R-HSA-2029481Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9674555Reactome: R-HSA-9680350Reactome: R-HSA-9705462Reactome: R-HSA-9706374 |
| Cellular Localisation | Isoform 1: LysosomeMembraneLipid-AnchorCell ProjectionPodosome MembraneCytoplasmCytosolAssociated With Specialized Secretory Lysosomes Called Azurophil GranulesAt Least Half Of This Isoform Is Found In The CytoplasmSome Of This Fraction Is MyristoylatedIsoform 2: Cell MembraneCaveolaCell JunctionFocal AdhesionCytoskeletonGolgi ApparatusCytoplasmic VesicleLysosomeNucleus20% Of This Isoform Is Associated With CaveolaeLocalization At The Cell Membrane And At Caveolae Requires Palmitoylation At Cys-3Colocalizes With The Actin Cytoskeleton At Focal AdhesionsSecretory Vesicle |
| Alternative Protein Names | Tyrosine-Protein Kinase Hck proteinHematopoietic Cell Kinase proteinHemopoietic Cell Kinase proteinP59-Hck/P60-Hck proteinP59hck proteinP61hck proteinHCK protein |
Information sourced from Uniprot.org