Human HB-EGF protein (Recombinant) (C-His) (STJP002661)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human HB-EGF Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Leu20-Leu148) of human HB-EGF (Accession #Q99075) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human HB-EGF-C-His protein was developed in hek293 cells using the region Leu20-Leu148. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.2 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.15-0.60 ng/mL, corresponding to a specific activity of 1.67×106~6.67×106 units/mg. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | HBEGF |
| Gene ID | 1839 |
| Uniprot ID | HBEGF_HUMAN |
| Immunogen | Recombinant Human HB-EGF Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Leu20-Leu148) of human HB-EGF (Accession #Q99075) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Leu20-Leu148 |
| Immunogen Sequence | LVTGESLERLRRGLAAGTSN PDPPTVSTDQLLPLGGGRDR KVRDLQEADLDLLRVTLSSK PQALATPNKEEHGKRKKKGK GLGKKRDPCLRKYKDFCIHG ECKYVKELRAPSCICHPGYH GERCHGLSL |
Additional Info
| Function | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. |
| Protein Name | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth FactorHb-EgfHbegfDiphtheria Toxin ReceptorDt-R |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1250342Reactome: R-HSA-1250347Reactome: R-HSA-1251985Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2179392Reactome: R-HSA-2219530Reactome: R-HSA-5336415Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8857538Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665686 |
| Cellular Localisation | Heparin-Binding Egf-Like Growth Factor: SecretedExtracellular SpaceMature Hb-Egf Is Released Into The Extracellular Space And Probably Binds To A ReceptorProheparin-Binding Egf-Like Growth Factor: Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor proteinHb-Egf proteinHbegf proteinDiphtheria Toxin Receptor proteinDt-R proteinHBEGF proteinDTR proteinDTS proteinHEGFL protein |
Information sourced from Uniprot.org