| Short Description : | Recombinant-Human Guanylate kinase 1/GUK1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications: | SDS-PAGE/Enzyme Activity |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 1 mg/mL |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 0.1M NaCl |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Specific activity is >100unit/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH. |
| Gene Symbol: | GUK1 |
| Gene ID: | 2987 |
| Uniprot ID: | KGUA_HUMAN |
| Accession Number: | NP_000849 |
| Immunogen: | Human |
| Immunogen Region: | 1-197aa |
| Immunogen Sequence: | MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA |
| Tissue Specificity | Widely expressed. |
| Function | Catalyzes the phosphorylation of GMP to GDP. Essential enzyme for recycling GMP and indirectly, cyclic GMP (cGMP). Involved in the cGMP metabolism in photoreceptors. It may also have a role in the survival and growth progression of some tumors. In addition to its physiological role, GUK1 is essential for converting prodrugs used for the treatment of cancers and viral infections into their pharmacologically active metabolites, most notably acyclovir, ganciclovir, and 6-thioguanine and its closely related analog 6-mercaptopurine. |
| Protein Name | Guanylate KinaseGmp KinaseGuanylate Kinase 1 |
| Database Links | Reactome: R-HSA-499943Reactome: R-HSA-9748787 |
| Cellular Localisation | Photoreceptor Inner SegmentCytoplasmCytosolColocalizes With Rd3 In Photoreceptor Inner Segments And To A Lesser Extent In The Outer Plexiform LayerIsoform 2: Mitochondrion |
| Alternative Protein Names | Guanylate Kinase proteinGmp Kinase proteinGuanylate Kinase 1 proteinGUK1 proteinGMK proteinGMPK protein |
Information sourced from Uniprot.org