| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | Please contact us for more information. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 1 Mu g/mL (100 Mu L/well) can bind Human Glypican 1 with a linear range of 2-80 ng/mL. |
| Gene Symbol: | GPC1 |
| Gene ID: | 2817 |
| Uniprot ID: | GPC1_HUMAN |
| Immunogen Region: | Asp24-Gln527 |
| Immunogen: | Recombinant Human Glypican-1/GPC1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp24-Gln527) of human Glypican 1 (Accession #NP_002072.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | DPASKSRSCGEVRQIYGAKG FSLSDVPQAEISGEHLRICP QGYTCCTSEMEENLANRSHA ELETALRDSSRVLQAMLATQ LRSFDDHFQHLLNDSERTLQ ATFPGAFGELYTQNARAFRD LYSELRLYYRGANLHLEETL AEFWARLLERLFKQLHPQLL LPDDYLDCLGKQAEALRPFG EAPRELRLRATRAFVAARSF VQGLGVASDVVRKVAQVPLG PECSRAVMKLVYCAHCLGV |
| Function | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. |
| Protein Name | Glypican-1 Cleaved Into - Secreted Glypican-1 |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022928Reactome: R-HSA-2024096Reactome: R-HSA-202733Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3656237Reactome: R-HSA-3656253Reactome: R-HSA-376176Reactome: R-HSA-4420332Reactome: R-HSA-9694614Reactome: R-HSA-975634Reactome: R-HSA-9820960Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular SideEndosomeS-Nitrosylated Form Recycled In EndosomesLocalizes To Cav1-Containing Vesicles Close To The Cell SurfaceCleavage Of Heparan Sulfate Side Chains Takes Place Mainly In Late EndosomesAssociates With Both Forms Of Prnp In Lipid RaftsColocalizes With App In Perinuclear Compartments And With Cp In Intracellular CompartmentsAssociates With Fibrillar App Amyloid-Beta Peptides In Lipid Rafts In Alzheimer Disease BrainsSecreted Glypican-1: SecretedExtracellular Space |
| Alternative Protein Names | Glypican-1 Cleaved Into - Secreted Glypican-1 proteinGPC1 protein |
Information sourced from Uniprot.org