Human GLI1 protein (Recombinant) (N-His) (STJP003823)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP003823
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General Information

Short DescriptionRecombinant-Human GLI1-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolGLI1
Gene ID2735
Uniprot IDGLI1_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionPhe2-Glu234

Additional Info

Post Translational Modifications Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity.
Function Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling. Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Protein Name Zinc Finger Protein Gli1
Glioma-Associated Oncogene
Oncogene Gli
Database Links Reactome: R-HSA-5610780
Reactome: R-HSA-5610787
Reactome: R-HSA-5632684
Reactome: R-HSA-5635851
Cellular Localisation Cytoplasm
Nucleus
Tethered In The Cytoplasm By Binding To Sufu
Activation And Translocation To The Nucleus Is Promoted By Interaction With Stk36
Phosphorylation By Ulk3 May Promote Nuclear Localization
Translocation To The Nucleus Is Promoted By Interaction With Zic1
Isoform 2: Cytoplasm
Alternative Protein Names Zinc Finger Protein Gli1 protein
Glioma-Associated Oncogene protein
Oncogene Gli protein
GLI1 protein
GLI protein

Information sourced from Uniprot.org

Citations

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