| Post Translational Modifications | Phosphorylated by PKN1. |
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Protein Name | Glial Fibrillary Acidic ProteinGfap |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-9613829 |
| Cellular Localisation | CytoplasmAssociated With Intermediate Filaments |
| Alternative ELISA Names | Glial Fibrillary Acidic Protein ELISA kitGfap ELISA kitGFAP ELISA kit |
| Specificity | This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-96 | 91 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 101-107 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.27 | 118.65 | 432.44 | 44.06 | 112.01 | 408.43 |
| Standard deviation | 2.46 | 5.54 | 19.98 | 2.41 | 5.35 | 22.26 |
| CV (%) | 5.96 | 4.67 | 4.62 | 5.47 | 4.78 | 5.45 |
