| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human GDF15 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GDF15 |
| Gene ID: |
9518 |
| Uniprot ID: |
GDF15_HUMAN |
| Specificity: |
This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level). |
| Function | Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling. |
| Protein Name | Growth/Differentiation Factor 15Gdf-15Macrophage Inhibitory Cytokine 1Mic-1Nsaid-Activated Gene 1 ProteinNag-1Nsaid-Regulated Gene 1 ProteinNrg-1Placental Tgf-BetaPlacental Bone Morphogenetic ProteinProstate Differentiation Factor |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Growth/Differentiation Factor 15 CLIA kitGdf-15 CLIA kitMacrophage Inhibitory Cytokine 1 CLIA kitMic-1 CLIA kitNsaid-Activated Gene 1 Protein CLIA kitNag-1 CLIA kitNsaid-Regulated Gene 1 Protein CLIA kitNrg-1 CLIA kitPlacental Tgf-Beta CLIA kitPlacental Bone Morphogenetic Protein CLIA kitProstate Differentiation Factor CLIA kitGDF15 CLIA kitMIC1 CLIA kitPDF CLIA kitPLAB CLIA kitPTGFB CLIA kit |
| Specificity | This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-112 | 107 |
| EDTA plasma(n=8) | 85-99 | 92 |
| Cell culture media(n=8) | 94-108 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.25 | 74.90 | 217.76 | 21.93 | 74.73 | 209.40 |
| Standard deviation | 2.26 | 8.80 | 24.82 | 2.43 | 7.88 | 21.71 |
| CV (%) | 9.32 | 11.75 | 11.40 | 11.08 | 10.54 | 10.37 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
