| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human GAP43 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GAP43 |
| Gene ID: |
2596 |
| Uniprot ID: |
NEUM_HUMAN |
| Specificity: |
This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
| Function | This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. |
| Protein Name | NeuromodulinAxonal Membrane Protein Gap-43Growth-Associated Protein 43Neural Phosphoprotein B-50Pp46 |
| Database Links | Reactome: R-HSA-373760 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionGrowth Cone MembraneSynapseFilopodium MembranePerikaryonDendriteAxonCytoplasmCytoplasmic Surface Of Growth Cone And Synaptic Plasma Membranes |
| Alternative CLIA Names | Neuromodulin CLIA kitAxonal Membrane Protein Gap-43 CLIA kitGrowth-Associated Protein 43 CLIA kitNeural Phosphoprotein B-50 CLIA kitPp46 CLIA kitGAP43 CLIA kit |
| Specificity | This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-112 | 106 |
| EDTA plasma(n=8) | 89-101 | 96 |
| Cell culture media(n=8) | 93-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.41 | 287.04 | 773.36 | 102.98 | 274.69 | 735.86 |
| Standard deviation | 7.59 | 25.03 | 49.96 | 10.74 | 32.47 | 61.52 |
| CV (%) | 8.04 | 8.72 | 6.46 | 10.43 | 11.82 | 8.36 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
