| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human GAD1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GAD1 |
| Gene ID: |
2571 |
| Uniprot ID: |
DCE1_HUMAN |
| Specificity: |
This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1: Expressed in brain. Isoform 3: Expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. |
| Function | Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3: Enzymatically inactive as glutamate decarboxylase. |
| Protein Name | Glutamate Decarboxylase 167 Kda Glutamic Acid DecarboxylaseGad-67Glutamate Decarboxylase 67 Kda Isoform |
| Database Links | Reactome: R-HSA-888568Reactome: R-HSA-888590Reactome: R-HSA-9022927 |
| Alternative CLIA Names | Glutamate Decarboxylase 1 CLIA kit67 Kda Glutamic Acid Decarboxylase CLIA kitGad-67 CLIA kitGlutamate Decarboxylase 67 Kda Isoform CLIA kitGAD1 CLIA kitGAD CLIA kitGAD67 CLIA kit |
| Specificity | This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-113 | 103 |
| EDTA plasma(n=8) | 98-114 | 105 |
| Cell culture media(n=8) | 102-114 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.99 | 2.40 | 9.36 | 1.01 | 2.23 | 10.04 |
| Standard deviation | 0.12 | 0.28 | 0.90 | 0.10 | 0.16 | 1.13 |
| CV (%) | 12.12 | 11.67 | 9.62 | 9.90 | 7.17 | 11.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
