| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human FGFR2 alpha (IIIc)-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | FGFR2 |
| Gene ID: | 2263 |
| Uniprot ID: | FGFR2_HUMAN |
| Immunogen Region: | Arg22-Glu377 |
| Immunogen: | DNA sequence encoding Human FGFR2 alpha (IIIc) including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. |
| Protein Name | Fibroblast Growth Factor Receptor 2 Fgfr-2 K-Sam Kgfr Keratinocyte Growth Factor Receptor Cd Antigen Cd332 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-190375 Reactome: R-HSA-190377 Reactome: R-HSA-2023837 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654221 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654727 Reactome: R-HSA-5655253 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851708 Reactome: R-HSA-8853333 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Golgi Apparatus Cytoplasmic Vesicle Detected On Osteoblast Plasma Membrane Lipid Rafts After Ligand Binding The Activated Receptor Is Rapidly Internalized And Degraded Isoform 1: Cell Membrane Isoform 3: Cell Membrane Isoform 8: Secreted Isoform 13: Secreted |
| Alternative Protein Names | Fibroblast Growth Factor Receptor 2 protein Fgfr-2 protein K-Sam protein Kgfr protein Keratinocyte Growth Factor Receptor protein Cd Antigen Cd332 protein FGFR2 protein BEK protein KGFR protein KSAM protein |
Information sourced from Uniprot.org