| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This FGF7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | FGF7 |
| Gene ID: | 2252 |
| Uniprot ID: | FGF7_HUMAN |
| Specificity: | This kit recognizes Human FGF7/KGF in samples. No significant cross-reactivity or interference between Human FGF7/KGF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. |
| Protein Name | Fibroblast Growth Factor 7Fgf-7Heparin-Binding Growth Factor 7Hbgf-7Keratinocyte Growth Factor |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-190377Reactome: R-HSA-2033519Reactome: R-HSA-2219530Reactome: R-HSA-5654221Reactome: R-HSA-5654695Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654727Reactome: R-HSA-5655253Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Fibroblast Growth Factor 7 ELISA kitFgf-7 ELISA kitHeparin-Binding Growth Factor 7 ELISA kitHbgf-7 ELISA kitKeratinocyte Growth Factor ELISA kitFGF7 ELISA kitKGF ELISA kit |
| Specificity | This kit recognizes Human FGF7/KGF in samples. No significant cross-reactivity or interference between Human FGF7/KGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-109 | 101 |
| EDTA plasma (n=8) | 94-111 | 102 |
| Cell culture media (n=8) | 85-97 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.69 | 217.61 | 889.61 | 96.83 | 210.35 | 850.06 |
| Standard deviation | 6.19 | 9.29 | 39.77 | 6.35 | 11.51 | 25.59 |
| CV (%) | 6.15 | 4.27 | 4.47 | 6.56 | 5.47 | 3.01 |