| Background | FCRN/FCGRT, also known as IgG receptor FcRn large subunit p51, is an IgG Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. This protein is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis. Also, it is expessed in neutrophils and myeloid antigen presenting cells. It is present in the placenta where it transports mother s IgG to the growing fetus. In addition to binding to IgG, it has been shown to interact with human serum albumin. FCRN/FCGRT-mediated transcytosis of IgG across epithelial cells is possible because FCRN binds IgG at acidic pH but not at neutral or higher pH. B2M, also known as beta 2-Microglobulin, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. It is a protein found on the surface of many cells and plentiful on the surface of white blood cells. B2M associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1, MR1, the neonatal Fc receptor (FCRN/FCGRT) , and Qa-1. Recombinant human FCGRT fused to His-tag at C-terminus, and B2M was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
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