| Short Description : | Recombinant-Human Fc-gamma RIII beta/CD16b-C-His protein was developed in hek293 cells using the region Thr20-Gln208. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD16 Antibody at 1 Mu g/mL (25 Mu L/well) can bind Human FCGR3B with a linear range of 0.46-1.16ng/mL. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | FCGR3B |
| Gene ID: | 2215 |
| Uniprot ID: | FCG3B_HUMAN |
| Immunogen: | Recombinant Human Fc-gamma RIII beta/CD16b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Gln208) of human Fc gamma RIIIB/CD16b (Accession #NP_000561.3) fused with a 6×His tag at the C-term |
| Immunogen Region: | Thr20-Gln208 |
| Immunogen Sequence: | TEDLPKAVVFLEPQWYSVLE KDSVTLKCQGAYSPEDNSTQ WFHNENLISSQASSYFIDAA TVNDSGEYRCQTNLSTLSDP VQLEVHIGWLLLQAPRWVFK EEDPIHLRCHSWKNTALHKV TYLQNGKDRKYFHHNSDFHI PKATLKDSGSYFCRGLVGSK NVSSETVNITITQGLAVSTI SSFSPPGYQ |
| Tissue Specificity | Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils. |
| Post Translational Modifications | Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3. The soluble form is produced by a proteolytic cleavage. |
| Function | Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
| Protein Name | Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-BFc-Gamma Riii-BetaCd16-IFc-Gamma RiiiFc-Gamma RiiibFcriiiFcriiibFcr-10Igg Fc Receptor Iii-1Cd Antigen Cd16b |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedSecreted After Cleavage |
| Alternative Protein Names | Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-B proteinFc-Gamma Riii-Beta proteinCd16-I proteinFc-Gamma Riii proteinFc-Gamma Riiib proteinFcriii proteinFcriiib proteinFcr-10 proteinIgg Fc Receptor Iii-1 proteinCd Antigen Cd16b proteinFCGR3B proteinCD16B proteinFCG3 proteinFCGR3 proteinIGFR3 protein |
Information sourced from Uniprot.org
