• Human ERK2/MAPK1 protein (Recombinant) (N-GST) (STJP000800)

Human ERK2/MAPK1 protein (Recombinant) (N-GST) (STJP000800)

SKU:
STJP000800

Current Stock:
Host: E. coli
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human ERK2/MAPK1-N-GST protein was developed from e. coli and has a target region of N-GST. For use in research applications.
Formulation: Lyophilized from a 0.22 Mu m filtered solution of 50mM TRIS, 150mM NaCl, 0.1mM EDTA, 0.25mM DTT, 0.1mM AEBSF, pH8.0. Contact us for customized product form or formulation.
Endotoxin: Please contact us for more information.
Gene Symbol: MAPK1
Gene ID: 5594
Uniprot ID: MK01_HUMAN
Immunogen: Recombinant Human ERK2/MAPK1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser360) of human MAPK1 (Accession #NP_002736.3) fused with a GST tag at the N-terminus.
Post Translational Modifications Phosphorylated upon KIT and FLT3 signaling. Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. Dephosphorylated by DUSP1 and DUSP2 at Thr-185 and Tyr-187 . ISGylated. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a GCAAAGC consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Protein Name Mitogen-Activated Protein Kinase 1
Map Kinase 1
Mapk 1
Ert1
Extracellular Signal-Regulated Kinase 2
Erk-2
Map Kinase Isoform P42
P42-Mapk
Mitogen-Activated Protein Kinase 2
Map Kinase 2
Mapk 2
Database Links Reactome: R-HSA-111995
Reactome: R-HSA-112409
Reactome: R-HSA-112411
Reactome: R-HSA-1181150
Reactome: R-HSA-1295596
Reactome: R-HSA-1502540
Reactome: R-HSA-162658
Reactome: R-HSA-170968
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2029482
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-2871796
Reactome: R-HSA-3371453
Reactome: R-HSA-375165
Reactome: R-HSA-437239
Reactome: R-HSA-444257
Reactome: R-HSA-445144
Reactome: R-HSA-450341
Reactome: R-HSA-456926
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5663213
Reactome: R-HSA-5668599
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6798695
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-6811558
Reactome: R-HSA-74749
Reactome: R-HSA-877300
Reactome: R-HSA-879415
Reactome: R-HSA-881907
Reactome: R-HSA-8939211
Reactome: R-HSA-8940973
Reactome: R-HSA-8943724
Reactome: R-HSA-9627069
Reactome: R-HSA-9634635
Reactome: R-HSA-9634638
Reactome: R-HSA-9635465
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9652817
Reactome: R-HSA-9656223
Reactome: R-HSA-9664422
Reactome: R-HSA-9725371
Reactome: R-HSA-9732724
Reactome: R-HSA-9768919
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Nucleus
Microtubule Organizing Center
Centrosome
Membrane
Caveola
Cell Junction
Focal Adhesion
Associated With The Spindle During Prometaphase And Metaphase
Pea15-Binding And Phosphorylated Dapk1 Promote Its Cytoplasmic Retention
Phosphorylation At Ser- 246 And Ser-248 As Well As Autophosphorylation At Thr-190 Promote Nuclear Localization
Alternative Protein Names Mitogen-Activated Protein Kinase 1 protein
Map Kinase 1 protein
Mapk 1 protein
Ert1 protein
Extracellular Signal-Regulated Kinase 2 protein
Erk-2 protein
Map Kinase Isoform P42 protein
P42-Mapk protein
Mitogen-Activated Protein Kinase 2 protein
Map Kinase 2 protein
Mapk 2 protein
MAPK1 protein
ERK2 protein
PRKM1 protein
PRKM2 protein

Information sourced from Uniprot.org

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