| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human ERCC2-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | ERCC2 |
| Gene ID: | 2068 |
| Uniprot ID: | ERCC2_HUMAN |
| Immunogen Region: | Ala404-Leu637 |
| Immunogen: | Homo sapiens (Human) |
| Function | ATP-dependent 5'-3' DNA helicase. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, not absolutely essential for minimal transcription in vitro. Required for transcription-coupled nucleotide excision repair (NER) of damaged DNA.recognizes damaged bases. Sequestered in chromatin on UV-damaged DNA. When complexed to CDK-activating kinase (CAK), involved in transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. Involved in DNA lesion verification. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex.large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA which allow this subunit to contact ssDNA. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase Subunit XpdTfiih Subunit XpdBasic Transcription Factor 2 80 Kda SubunitBtf2 P80CxpdDna 5'-3' Helicase XpdDna Excision Repair Protein Ercc-2Dna Repair Protein Complementing Xp-D CellsTfiih Basal Transcription Factor Complex 80 Kda SubunitTfiih 80 Kda SubunitTfiih P80Xeroderma Pigmentosum Group D-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-2564830Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative Protein Names | General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd proteinTfiih Subunit Xpd proteinBasic Transcription Factor 2 80 Kda Subunit proteinBtf2 P80 proteinCxpd proteinDna 5'-3' Helicase Xpd proteinDna Excision Repair Protein Ercc-2 proteinDna Repair Protein Complementing Xp-D Cells proteinTfiih Basal Transcription Factor Complex 80 Kda Subunit proteinTfiih 80 Kda Subunit proteinTfiih P80 proteinXeroderma Pigmentosum Group D-Complementing Protein proteinERCC2 proteinXPD proteinXPDC protein |
Information sourced from Uniprot.org