| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Ephrin-A1/EFNA1-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human EFNA1 at 1 Mu g/mL (100 Mu L/well) can bind Human Ephrin A1 Rabbit monoclonal antibody with a linear range of 0.098-2.066 ng/mL. |
| Gene Symbol: |
EFNA1 |
| Gene ID: |
1942 |
| Uniprot ID: |
EFNA1_HUMAN |
| Immunogen Region: |
Asp19-Ser182 |
| Immunogen: |
Recombinant Human Ephrin-A1/EFNA1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp19-Ser182) of human Ephrin-A1/EFNA1 (Accession #NP_004419.2) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form. N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization. |
| Function | Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. |
| Protein Name | Ephrin-A1Eph-Related Receptor Tyrosine Kinase Ligand 1Lerk-1Immediate Early Response Protein B61Tumor Necrosis Factor Alpha-Induced Protein 4Tnf Alpha-Induced Protein 4 Cleaved Into - Ephrin-A1 - Secreted Form |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorEphrin-A1Secreted Form: Secreted |
| Alternative Protein Names | Ephrin-A1 proteinEph-Related Receptor Tyrosine Kinase Ligand 1 proteinLerk-1 proteinImmediate Early Response Protein B61 proteinTumor Necrosis Factor Alpha-Induced Protein 4 proteinTnf Alpha-Induced Protein 4 Cleaved Into - Ephrin-A1 - Secreted Form proteinEFNA1 proteinEPLG1 proteinLERK1 proteinTNFAIP4 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
