Human EGLN1 protein (Recombinant) (N-His) (STJP007854)

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STJP007854
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Host: E. coli
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Human EGLN1-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications.
Formulation: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage Instruction: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.
Gene Symbol: EGLN1
Gene ID: 54583
Uniprot ID: EGLN1_HUMAN
Immunogen Region: Ala190-Phe426
Immunogen: Homo sapiens (Human)
Post Translational Modifications S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326.
Function Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
Protein Name Egl Nine Homolog 1
Hypoxia-Inducible Factor Prolyl Hydroxylase 2
Hif-Ph2
Hif-Prolyl Hydroxylase 2
Hph-2
Prolyl Hydroxylase Domain-Containing Protein 2
Phd2
Sm-20
Database Links Reactome: R-HSA-1234176
Cellular Localisation Cytoplasm
Nucleus
Mainly Cytoplasmic
Shuttles Between The Nucleus And Cytoplasm
Nuclear Export Requires Functional Xpo1
Alternative Protein Names Egl Nine Homolog 1 protein
Hypoxia-Inducible Factor Prolyl Hydroxylase 2 protein
Hif-Ph2 protein
Hif-Prolyl Hydroxylase 2 protein
Hph-2 protein
Prolyl Hydroxylase Domain-Containing Protein 2 protein
Phd2 protein
Sm-20 protein
EGLN1 protein
C1orf12 protein
PNAS-118 protein
PNAS-137 protein

Information sourced from Uniprot.org