Human EFNB1 protein (Recombinant-Active) (C-hFc-His) (STJP012055)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human EFNB1-C-hFc-His protein was developed from mammalian cells and has a target region of C-hFc-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | When recombinant Mouse EphB3 is coated at 2 Mu g/mL, recombinant human EFNB1 binds with an apparent KD <0.2 nM. |
Target Information
| Gene Symbol | EFNB1 |
| Gene ID | 1947 |
| Uniprot ID | EFNB1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu28-Gly232 |
Additional Info
| Post Translational Modifications | Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. Proteolytically processed. The ectodomain is cleaved, probably by a metalloprotease, to produce a membrane-tethered C-terminal fragment. This fragment is then further processed by the gamma-secretase complex to yield a soluble intracellular domain peptide which can translocate to the nucleus. The intracellular domain peptide is highly labile suggesting that it is targeted for degradation by the proteasome. |
| Function | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. |
| Protein Name | Ephrin-B1Efl-3Elk LigandElk-LEph-Related Receptor Tyrosine Kinase Ligand 2Lerk-2 Cleaved Into - Ephrin-B1 C-Terminal FragmentEphrin-B1 Ctf - Ephrin-B1 Intracellular DomainEphrin-B1 Icd |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftMay Recruit Grip1 And Grip2 To Membrane Raft DomainsEphrin-B1 C-Terminal Fragment: Cell MembraneEphrin-B1 Intracellular Domain: NucleusColocalizes With Zhx2 In The Nucleus |
| Alternative Protein Names | Ephrin-B1 proteinEfl-3 proteinElk Ligand proteinElk-L proteinEph-Related Receptor Tyrosine Kinase Ligand 2 proteinLerk-2 Cleaved Into - Ephrin-B1 C-Terminal Fragment proteinEphrin-B1 Ctf - Ephrin-B1 Intracellular Domain proteinEphrin-B1 Icd proteinEFNB1 proteinEFL3 proteinEPLG2 proteinLERK2 protein |
Information sourced from Uniprot.org