| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.054ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description : | This E2F1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | E2F1 |
| Gene ID: | 1869 |
| Uniprot ID: | E2F1_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Phosphorylation at Ser-403 by GSK3B promotes interaction with USP11, leading to its deubiquitination and stabilization. Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation. Deubiquitinated by USP11 following phosphorylation by GSK3B, promoting its stability. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B. |
| Protein Name | Transcription Factor E2f1E2f-1Pbr3Retinoblastoma-Associated Protein 1Rbap-1Retinoblastoma-Binding Protein 3Rbbp-3Prb-Binding Protein E2f-1 |
| Database Links | Reactome: R-HSA-111448Reactome: R-HSA-113501Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-139915Reactome: R-HSA-1912408Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-6804116Reactome: R-HSA-68911Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656Reactome: R-HSA-8953750Reactome: R-HSA-9616222Reactome: R-HSA-9661069 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Transcription Factor E2f1 ELISA kitE2f-1 ELISA kitPbr3 ELISA kitRetinoblastoma-Associated Protein 1 ELISA kitRbap-1 ELISA kitRetinoblastoma-Binding Protein 3 ELISA kitRbbp-3 ELISA kitPrb-Binding Protein E2f-1 ELISA kitE2F1 ELISA kitRBBP3 ELISA kit |
| output |
Information sourced from Uniprot.org
