| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Supplied as a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >4500 pmol/min/Mu g. |
| Gene Symbol: | DPP4 |
| Gene ID: | 1803 |
| Uniprot ID: | DPP4_HUMAN |
| Immunogen Region: | Ser39-Pro766 |
| Immunogen: | Recombinant Human DPP4/CD26 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser39-Pro766) of human DPP4/CD26 (Accession #NP_001926.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | SRKTYTLTDYLKNTYRLKLY SLRWISDHEYLYKQENNILV FNAEYGNSSVFLENSTFDEF GHSINDYSISPDGQFILLEY NYVKQWRHSYTASYDIYDLN KRQLITEERIPNNTQWVTWS PVGHKLAYVWNNDIYVKIEP NLPSYRITWTGKEDIIYNGI TDWVYEEEVFSAYSALWWSP NGTFLAYAQFNDTEVPLIEY SFYSDESLQYPKTVRVPYPK AGAVNPTVKFFVVNTDSLS |
| Post Translational Modifications | The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. |
| Function | Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2. |
| Protein Name | Dipeptidyl Peptidase 4 Adabp Adenosine Deaminase Complexing Protein 2 Adcp-2 Dipeptidyl Peptidase Iv Dpp Iv T-Cell Activation Antigen Cd26 Tp103 Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form Dipeptidyl Peptidase Iv Soluble Form |
| Database Links | Reactome: R-HSA-381771 Reactome: R-HSA-400511 |
| Cellular Localisation | Dipeptidyl Peptidase 4 Soluble Form: Secreted Detected In The Serum And The Seminal Fluid Cell Membrane Single-Pass Type Ii Membrane Protein Apical Cell Membrane Cell Projection Invadopodium Membrane Lamellipodium Membrane Cell Junction Membrane Raft Translocated To The Apical Membrane Through The Concerted Action Of N- And O-Glycans And Its Association With Lipid Microdomains Containing Cholesterol And Sphingolipids Redistributed To Membrane Rafts In T-Cell In An Interleukin-12-Dependent Activation Its Interaction With Cav1 Is Necessary For Its Translocation To Membrane Rafts Colocalized With Ptprc In Membrane Rafts Colocalized With Fap In Invadopodia And Lamellipodia Of Migratory Activated Endothelial Cells In Collagenous Matrix Colocalized With Fap On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal Carcinoma Colocalized With Ada At The Cell Junction In Lymphocyte-Epithelial Cell Adhesion Colocalized With Igf2r In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface |
| Alternative Protein Names | Dipeptidyl Peptidase 4 protein Adabp protein Adenosine Deaminase Complexing Protein 2 protein Adcp-2 protein Dipeptidyl Peptidase Iv protein Dpp Iv protein T-Cell Activation Antigen Cd26 protein Tp103 protein Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form protein Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form protein Dipeptidyl Peptidase Iv Soluble Form protein DPP4 protein ADCP2 protein CD26 protein |
Information sourced from Uniprot.org