| Host: |
E.coli |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Supplied as 0.22 Mu m filtered solution in 25mM Hepes-NaOH, 500mM NaCl, 0.04%, tritonX 100, 0.5mM TCEP, 10%Glycerol, pH 7.5. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
<1 EU/Mu g |
| Immunoreactivity: |
The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 µM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 µM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, a |
| Gene Symbol: |
DNMT1 |
| Gene ID: |
1786 |
| Uniprot ID: |
DNMT1_HUMAN |
| Immunogen Region: |
Pro2-Asp1632 |
| Immunogen: |
Recombinant Human DNMT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro2-Asp1632) of Human DNMT1 (Accession #NP_001124295.1) fused with a His tag at the C-terminus. |
| Immunogen Sequence: |
AKHKQRKRLKSSCKRHPLYV DFSDVGWNDWIVAPPGYHAF YCHGECPFPLADHLNSTNHA IVQTLVNSVNSKIPKACCVP TELSAISMLYLDENEKVVLK NYQDMVVEGCGCR |
| Tissue Specificity | Ubiquitous.highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. |
| Post Translational Modifications | Sumoylated.sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation. Ubiquitinated by UHRF1.interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
| Function | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth. |
| Protein Name | DnaCytosine-5-Methyltransferase 1Dnmt1Cxxc-Type Zinc Finger Protein 9Dna Methyltransferase HsaiDna Mtase HsaiM.hsaiMcmt |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-427413Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9701898Reactome: R-HSA-9710421Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusLocalized To The Perinucleolar Region |
| Alternative Protein Names | Dna proteinCytosine-5-Methyltransferase 1 proteinDnmt1 proteinCxxc-Type Zinc Finger Protein 9 proteinDna Methyltransferase Hsai proteinDna Mtase Hsai proteinM.hsai proteinMcmt proteinDNMT1 proteinAIM proteinCXXC9 proteinDNMT protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
