| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human DKK1-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | DKK1 |
| Gene ID: | 22943 |
| Uniprot ID: | DKK1_HUMAN |
| Immunogen Region: | Thr32-His266 |
| Immunogen: | DNA sequence encoding Human DKK1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. |
| Protein Name | Dickkopf-Related Protein 1Dickkopf-1Dkk-1Hdkk-1Sk |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 1 proteinDickkopf-1 proteinDkk-1 proteinHdkk-1 proteinSk proteinDKK1 proteinUNQ492 proteinPRO1008 protein |
Information sourced from Uniprot.org