• Biotinylated Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Biotinylated Human DKK1, His Tag at 5 Mu g/ml (100 Mu l/well) on the streptavidin precoated plate (5 Mu g/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3. 2ug/ml determined by ELISA.

Human DKK1 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001751)

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STJP001751

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human DKK1-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: DKK1
Gene ID: 22943
Uniprot ID: DKK1_HUMAN
Immunogen Region: Thr32-His266
Immunogen: DNA sequence encoding Human DKK1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity.
Protein Name Dickkopf-Related Protein 1
Dickkopf-1
Dkk-1
Hdkk-1
Sk
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Reactome: R-HSA-5339717
Cellular Localisation Secreted
Alternative Protein Names Dickkopf-Related Protein 1 protein
Dickkopf-1 protein
Dkk-1 protein
Hdkk-1 protein
Sk protein
DKK1 protein
UNQ492 protein
PRO1008 protein

Information sourced from Uniprot.org

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