| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.051ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This DIAPH1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | DIAPH1 |
| Gene ID: | 1729 |
| Uniprot ID: | DIAP1_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets. |
| Post Translational Modifications | Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement. |
| Function | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Plays a role in brain development. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity. |
| Protein Name | Protein Diaphanous Homolog 1 Diaphanous-Related Formin-1 Drf1 |
| Database Links | Reactome: R-HSA-5663220 Reactome: R-HSA-6785631 Reactome: R-HSA-6798695 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013405 Reactome: R-HSA-9035034 Reactome: R-HSA-9854909 |
| Cellular Localisation | Cell Membrane Cell Projection Ruffle Membrane Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Spindle Nucleus Membrane Ruffles Especially At The Tip Of Ruffles Of Motile Cells |
| Alternative ELISA Names | Protein Diaphanous Homolog 1 ELISA kit Diaphanous-Related Formin-1 ELISA kit Drf1 ELISA kit DIAPH1 ELISA kit DIAP1 ELISA kit |
| output |
Information sourced from Uniprot.org
