Human D-LDH (D-Lactate Dehydrogenase) Sandwich ELISA Kit (STJE0002540)

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STJE0002540
£163.50 - £357.50
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description : This D-LDH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LDHD
Gene ID: 197257
Uniprot ID: LDHD_HUMAN
Specificity: This kit recognizes Human D-LDH in samples. No significant cross-reactivity or interference between Human D-LDH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Involved in D-lactate, but not L-lactate catabolic process.
Protein Name Probable D-Lactate Dehydrogenase - Mitochondrial
Dld
Lactate Dehydrogenase D
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion
Alternative ELISA Names Probable D-Lactate Dehydrogenase - Mitochondrial ELISA kit
Dld ELISA kit
Lactate Dehydrogenase D ELISA kit
LDHD ELISA kit
Specificity This kit recognizes Human D-LDH in samples. No significant cross-reactivity or interference between Human D-LDH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-97 90
EDTA plasma (n=8) 84-100 91
Cell culture media (n=8) 87-98 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 5.00 15.80 36.60 4.50 14.30 39.90
Standard deviation 0.30 0.90 1.70 0.20 0.70 1.40
CV (%) 6.00 5.70 4.64 4.44 4.90 3.51

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