• Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD28 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-CD28 Antibody, hFc Tag at 2 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24. 4ng/ml determined by ELISA.

Human/Cynomolgus/Rhesus macaque CD28 protein (Recombinant) (N-His-Avi) {Biotin} (STJP001589)

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STJP001589

£597.50 - £1,839.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human/Cynomolgus/Rhesus macaque CD28-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD28
Gene ID: 940
Uniprot ID: CD28_HUMAN
Immunogen Region: Asn19-Pro152
Immunogen: DNA sequence encoding Human/Cynomolgus/Rhesus macaque CD28 including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Phosphorylated by LCK. Dephosphorylated by PTPN11. Isoform 3: Tyrosine phosphorylated induced by CD40LG.
Function Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. Isoform 3: Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.
Protein Name T-Cell-Specific Surface Glycoprotein Cd28
Tp44
Cd Antigen Cd28
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-164939
Reactome: R-HSA-2219530
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Cell Surface
Alternative Protein Names T-Cell-Specific Surface Glycoprotein Cd28 protein
Tp44 protein
Cd Antigen Cd28 protein
CD28 protein

Information sourced from Uniprot.org

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