| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human CXCL8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL8 |
| Gene ID: |
3576 |
| Uniprot ID: |
IL8_HUMAN |
| Specificity: |
This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP.leading to impaired neutrophil endothelial transmigration and thus increased virulence. |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Interleukin-8 CLIA kitIl-8 CLIA kitC-X-C Motif Chemokine 8 CLIA kitChemokine CLIA kitC-X-C Motif Ligand 8 CLIA kitEmoctakin CLIA kitGranulocyte Chemotactic Protein 1 CLIA kitGcp-1 CLIA kitMonocyte-Derived Neutrophil Chemotactic Factor CLIA kitMdncf CLIA kitMonocyte-Derived Neutrophil-Activating Peptide CLIA kitMonap CLIA kitNeutrophil-Activating Protein 1 CLIA kitNap-1 CLIA kitProtein 3-10c CLIA kitT-Cell Chemotactic Factor Cleaved Into - Mdncf-A CLIA kitGcp/Il-8 Protein Iv CLIA kitIl8/Nap1 Form I - Interleukin-8 CLIA kit(Ala-Il-877 CLIA kitGcp/Il-8 Protein Ii CLIA kitIl-8(1-77 CLIA kitIl8/Nap1 Form Ii CLIA kitMdncf-B - Il-8(5-77 - Il-8(6-77 CLIA kit(Ser-Il-872 CLIA kitGcp/Il-8 Protein I CLIA kitIl8/Nap1 Form Iii CLIA kitLymphocyte-Derived Neutrophil-Activating Factor CLIA kitLynap CLIA kitMdncf-C CLIA kitNeutrophil-Activating Factor CLIA kitNaf - Il-8(7-77 CLIA kitGcp/Il-8 Protein V CLIA kitIl8/Nap1 Form Iv - Il-8(8-77 CLIA kitGcp/Il-8 Protein Vi CLIA kitIl8/Nap1 Form V - Il-8(9-77 CLIA kitGcp/Il-8 Protein Iii CLIA kitIl8/Nap1 Form Vi CLIA kitCXCL8 CLIA kitIL8 CLIA kit |
| Specificity | This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma(n=8) | 100-115 | 107 |
| Cell culture media(n=8) | 99-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.63 | 106.84 | 411.53 | 48.85 | 107.69 | 421.00 |
| Standard deviation | 4.97 | 11.51 | 28.64 | 5.72 | 8.63 | 28.50 |
| CV (%) | 9.82 | 10.77 | 6.96 | 11.71 | 8.01 | 6.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
