Human CXCL10 (C-X-C motif chemokine 10) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human CXCL10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 4.69pg/mL |
| Detection Limit | 7.81~500pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | CXCL10 |
| Gene ID | 3627 |
| Uniprot ID | CXL10_HUMAN |
| Specificity | This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation. |
| Post Translational Modifications | Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead. |
| Function | Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization. |
| Protein Name | C-X-C Motif Chemokine 1010 Kda Interferon Gamma-Induced ProteinGamma-Ip10Ip-10Small-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 10 CLIA kit10 Kda Interferon Gamma-Induced Protein CLIA kitGamma-Ip10 CLIA kitIp-10 CLIA kitSmall-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 CLIA kitCXCL10 CLIA kitINP10 CLIA kitSCYB10 CLIA kit |
| Specificity | This kit recognizes Human IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-111 | 102 |
| EDTA plasma(n=8) | 100-115 | 107 |
| Cell culture media(n=8) | 85-96 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.60 | 72.26 | 192.19 | 24.27 | 73.23 | 175.93 |
| Standard deviation | 2.01 | 6.56 | 15.34 | 2.60 | 8.41 | 17.43 |
| CV (%) | 8.17 | 9.08 | 7.98 | 10.71 | 11.48 | 9.91 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human CXCL10 (C-X-C motif chemokine 10) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect human CXCL10 (also known as IP-10) in serum, plasma, and other biological fluids. It is validated for human samples only, with no significant cross-reactivity observed between human CXCL10 and analogues.
The detection range is 7.81 to 500 pg/mL, with a sensitivity of 4.69 pg/mL. Samples with CXCL10 concentrations outside this range may require dilution or may fall below the limit of detection.
This kit uses chemiluminescence (CLIA) for signal detection, which is the basis for its quantitative readout of human CXCL10 in biological samples.
The total assay time is approximately 3.5 hours from start to finish.
If unopened, the kit can be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month, components should be stored separately according to the component storage table provided in the manual.
No, this kit is for research use only and is not intended for diagnostic, therapeutic, or clinical laboratory procedures.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.