| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.118ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This CUL9 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
CUL9 |
| Gene ID: |
23113 |
| Uniprot ID: |
CUL9_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Ubiquitously expressed in all tissues with highest expression in testis brain and kidney. |
| Post Translational Modifications | Autoubiquitinated by the CUL9-RBX1 complex at Lys-87. Neddylated. Neddylation is mediated by E1 enzyme UBA3-NAE1 complex and E2 enzyme UBE2F. Structural rearrangment of the C-terminal TRIAD/RBR supradomain may play a role in neddylation and deneddylation. |
| Function | Core component of a Cul9-RING ubiquitin-protein ligase complex composed of CUL9 and RBX1. The CUL9-RBX1 complex mediates ubiquitination and subsequent degradation of BIRC5 and is required to maintain microtubule dynamics and genome integrity. Acts downstream of the 3M complex, which inhibits the ubiquitination of BIRC5. The CUL9-RBX1 complex also mediates mono-ubiquitination of p53/TP53. Acts as a cytoplasmic anchor protein in p53/TP53-associated protein complex. Regulates the subcellular localization of p53/TP53 and its subsequent function. Ubiquitinates apurinic/apyrimidinic endodeoxyribonuclease APEX2. Ubiquitination by the CUL9-RBX1 complex is predominantly mediated by E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2D2. |
| Protein Name | Cullin-9Cul-9Ubch7-Associated Protein 1P53-Associated Parkin-Like Cytoplasmic Protein |
| Database Links | Reactome: R-HSA-8951664 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Cullin-9 ELISA kitCul-9 ELISA kitUbch7-Associated Protein 1 ELISA kitP53-Associated Parkin-Like Cytoplasmic Protein ELISA kitCUL9 ELISA kitH7AP1 ELISA kitKIAA0708 ELISA kitPARC ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
