• 96-well maxisorp plates were coated at 100ng/well recombinant human B7-1 Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C. Absorbance was measured at 450-615 nm using an automated plate reader.

Human CTLA4 (CD152) protein (Recombinant) {Biotin} (STJP000202)

SKU:
STJP000202

£412.50 - £2,057.50
Shipping:
Free Shipping
Current Stock:
Host: HEK293
Applications: ELISA/WB
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human CTLA4 (CD152)-protein was developed from hek293. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 Mu m filtered PBS pH 7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Gene Symbol: CTLA4
Gene ID: 1493
Uniprot ID: CTLA4_HUMAN
Immunogen Region: ECD
Immunogen: DNA sequence encoding extracellular domain of Human CTLA4 to a C-terminal polyHis tag was expressed in HEK cells. NA
Tissue Specificity Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
Post Translational Modifications N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Protein Name Cytotoxic T-Lymphocyte Protein 4
Cytotoxic T-Lymphocyte-Associated Antigen 4
Ctla-4
Cd Antigen Cd152
Database Links Reactome: R-HSA-389513
Reactome: R-HSA-8877330
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Exists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization
Alternative Protein Names Cytotoxic T-Lymphocyte Protein 4 protein
Cytotoxic T-Lymphocyte-Associated Antigen 4 protein
Ctla-4 protein
Cd Antigen Cd152 protein
CTLA4 protein
CD152 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance