Human Cortisol (Cortisol) Competitive ELISA Kit (STJE0001911)
SPECIFICATIONS
General Information
| Short Description | This Cortisol Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, urine and saliva. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 2.92ng/mL |
| Detection Limit | 6.25~400ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 2.5h |
| Detection | Colormetric |
Target Information
| Specificity | This kit recognizes Human Cortisol in samples. No significant cross-reactivity or interference between Human Cortisol and analogues was observed. |
| Sample Type | Serum, Plasma, Urine and Saliva |
Additional Info
| Specificity | This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 81-93 | 85 |
| EDTA plasma(n=8) | 86-96 | 89 |
| Urine(n=8) | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 50.33 | 100.32 | 200.52 | 51.27 | 99.84 | 202.18 |
| Standard deviation | 4.42 | 8.08 | 14.90 | 4.59 | 7.02 | 14.46 |
| CV (%) | 8.78 | 8.05 | 7.43 | 8.96 | 7.03 | 7.15 |