| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This human COL1A2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | COL1A2 |
| Gene ID: | 1278 |
| Uniprot ID: | CO1A2_HUMAN |
| Specificity: | This kit recognizes Human COL1 Alpha 2 in samples. No significant cross-reactivity or interference between Human COL1 Alpha 2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Protein Name | Collagen Alpha-2(I Chain Alpha-2 Type I Collagen |
| Database Links | Reactome: R-HSA-114604 Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2173796 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-430116 Reactome: R-HSA-6785807 Reactome: R-HSA-75892 Reactome: R-HSA-76009 Reactome: R-HSA-8874081 Reactome: R-HSA-8948216 Reactome: R-HSA-9845619 Reactome: R-HSA-9845620 Reactome: R-HSA-9845621 Reactome: R-HSA-9845622 Reactome: R-HSA-9846298 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative CLIA Names | Collagen Alpha-2(I Chain CLIA kit Alpha-2 Type I Collagen CLIA kit COL1A2 CLIA kit |
| Specificity | This kit recognizes Human COL1α2 in samples. No significant cross-reactivity or interference between Human COL1α2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 99-114 | 107 |
| EDTA plasma(n=8) | 86-96 | 91 |
| Cell culture media(n=8) | 89-101 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.56 | 189.05 | 753.17 | 109.77 | 198.00 | 744.85 |
| Standard deviation | 12.43 | 16.07 | 48.73 | 12.43 | 14.59 | 64.36 |
| CV (%) | 12.00 | 8.50 | 6.47 | 11.32 | 7.37 | 8.64 |