| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cell line. The ED50 for this effect is 13.5-54 ng/mL. |
| Gene Symbol: | CNTF |
| Gene ID: | 1270 |
| Uniprot ID: | CNTF_HUMAN |
| Immunogen Region: | Met1-Met200 |
| Immunogen: | Recombinant Human CNTF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Met200) of human CNTF (Accession #NP_000605.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | MAFTEHSPLTPHRRDLCSRS IWLARKIRSDLTALTESYVK HQGLNKNINLDSADGMPVAS TDQWSELTEAERLQENLQAY RTFHVLLARLLEDQQVHFTP TEGDFHQAIHTLLLQVAAFA YQIEELMILLEYKIPRNEAD GMPINVGDGGLFEKKLWGLK VLQELSQWTVRSIHDLRFIS SHQTGIPARGSHYIANNKKM |
| Tissue Specificity | Nervous system. |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. |
| Protein Name | Ciliary Neurotrophic FactorCntf |
| Database Links | Reactome: R-HSA-6788467 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Ciliary Neurotrophic Factor proteinCntf proteinCNTF protein |
Information sourced from Uniprot.org