Protein Name | Creatine Kinase M-TypeCreatine Kinase M ChainCreatine Phosphokinase M-TypeCpk-MM-Ck |
Database Links | Reactome: R-HSA-71288 |
Cellular Localisation | Cytoplasm |
Alternative ELISA Names | Creatine Kinase M-Type ELISA kitCreatine Kinase M Chain ELISA kitCreatine Phosphokinase M-Type ELISA kitCpk-M ELISA kitM-Ck ELISA kitCKM ELISA kitCKMM ELISA kit |
Specificity | This kit recognizes Human CKMB in samples. No significant cross-reactivity or interference between Human CKMB and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 92-105 | 98 |
EDTA plasma (n=8) | 93-107 | 101 |
Cell culture media (n=8) | 91-104 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 25.24 | 67.45 | 236.70 | 24.33 | 72.18 | 220.81 |
Standard deviation | 1.02 | 2.86 | 9.32 | 1.00 | 3.29 | 10.43 |
CV (%) | 4.04 | 4.24 | 3.93 | 4.11 | 4.56 | 4.72 |