| Function | Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases. CFB is cleaved and activated by CFD to generate Ba and Bb chains.Bb chain constituting the catalytic component of the C3 and C5 convertases. Complement factor B Bb: Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway. Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b. As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways. When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex. Complement factor B Ba: Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. |
| Protein Name | Complement Factor BC3/C5 ConvertaseGlycine-Rich Beta GlycoproteinGbgPbf2Properdin Factor B Cleaved Into - Complement Factor B BaBa - Complement Factor B BbBb |
| Database Links | Reactome: R-HSA-173736Reactome: R-HSA-174577Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedComplement Factor B Bb: Cell SurfaceRecruited To The Surface Of Pathogens By Complement C3b Opsonin |
| Alternative ELISA Names | Complement Factor B ELISA kitC3/C5 Convertase ELISA kitGlycine-Rich Beta Glycoprotein ELISA kitGbg ELISA kitPbf2 ELISA kitProperdin Factor B Cleaved Into - Complement Factor B Ba ELISA kitBa - Complement Factor B Bb ELISA kitBb ELISA kitCFB ELISA kitBF ELISA kitBFD ELISA kit |
| Specificity | This kit recognizes Human CFB in samples. No significant cross-reactivity or interference between Human CFB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-107 | 104 |
| EDTA plasma (n=8) | 98-107 | 103 |
| Cell culture media (n=8) | 98-109 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.81 | 2.18 | 8.88 | 0.84 | 2.03 | 9.20 |
| Standard deviation | 0.05 | 0.10 | 0.46 | 0.04 | 0.10 | 0.44 |
| CV (%) | 6.17 | 4.59 | 5.18 | 4.76 | 4.93 | 4.78 |
