Human CEACAM5 (Carcinoembryonic antigen-related cell adhesion molecule 5) Sandwich ELISA Kit (STJE0006757)

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STJE0006757
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78-50ng/mL
Short Description : This CEACAM5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum and plasma.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: CEACAM5
Gene ID: 1048
Uniprot ID: CEAM5_HUMAN
Specificity: This kit recognizes Human CEA in samples. No significant cross-reactivity or interference between Human CEA and analogues was observed.
Sample Type: Serum and plasma
Post Translational Modifications Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Function Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression.induces resistance to anoikis of colorectal carcinoma cells. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Protein Name Cell Adhesion Molecule Ceacam5
Carcinoembryonic Antigen
Cea
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5
Cea Cell Adhesion Molecule 5
Meconium Antigen 100
Cd Antigen Cd66e
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Apical Cell Membrane
Cell Surface
Localized To The Apical Glycocalyx Surface
Alternative ELISA Names Cell Adhesion Molecule Ceacam5 ELISA kit
Carcinoembryonic Antigen ELISA kit
Cea ELISA kit
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 ELISA kit
Cea Cell Adhesion Molecule 5 ELISA kit
Meconium Antigen 100 ELISA kit
Cd Antigen Cd66e ELISA kit
CEACAM5 ELISA kit
CEA ELISA kit
Specificity This kit recognizes CEA in samples. No significant cross-reactivity or interference between CEA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 100
EDTA plasma(n=8) 87-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.44 7.17 18.89 2.29 6.53 19.86
Standard deviation 0.13 0.40 0.60 0.13 0.26 0.66
CV (%) 5.16 5.51 3.16 5.79 4.01 3.32

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