Human CDC34 protein (Recombinant) (N-His) (STJP006630)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CDC34-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CDC34 |
| Gene ID | 997 |
| Uniprot ID | UB2R1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Ser236 |
Additional Info
| Post Translational Modifications | Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway. Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controls the nuclear localization. |
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 R1(E3-Independent E2 Ubiquitin-Conjugating Enzyme R1E2 Ubiquitin-Conjugating Enzyme R1Ubiquitin-Conjugating Enzyme E2-32 Kda ComplementingUbiquitin-Conjugating Enzyme E2-Cdc34Ubiquitin-Protein Ligase R1 |
| Database Links | Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-5607764Reactome: R-HSA-8866652Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusThe Phosphorylation Of The C-Terminal Tail Plays An Important Role In Mediating Nuclear LocalizationColocalizes With Beta-Tubulin On Mitotic Spindles In Anaphase |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 R1 protein(E3-Independent E2 Ubiquitin-Conjugating Enzyme R1 proteinE2 Ubiquitin-Conjugating Enzyme R1 proteinUbiquitin-Conjugating Enzyme E2-32 Kda Complementing proteinUbiquitin-Conjugating Enzyme E2-Cdc34 proteinUbiquitin-Protein Ligase R1 proteinCDC34 proteinUBCH3 proteinUBE2R1 protein |
Information sourced from Uniprot.org