Human CD8B protein (Recombinant) (C-His) (STJP013170)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CD8B-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 °C for one week. Store at-20 °C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | CD8B |
| Gene ID | 926 |
| Uniprot ID | CD8B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Pro170 |
Additional Info
| Post Translational Modifications | Phosphorylated as a consequence of T-cell activation. Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Beta ChainCd Antigen Cd8b |
| Database Links | Reactome: R-HSA-182218Reactome: R-HSA-198933 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinRequires The Partner Cd8a For Efficient Cell Surface ExpressionThe Heterodimer Cd8a/Cd8b Localizes To Lipid Rafts Due To Cd8b Cytoplasmic Tail PalmitoylationIsoform 2: Cell MembraneIsoform 3: SecretedIsoform 4: Cell MembraneIsoform 5: Cell MembraneIsoform 6: SecretedIsoform 7: SecretedIsoform 8: Secreted |
| Alternative Protein Names | T-Cell Surface Glycoprotein Cd8 Beta Chain proteinCd Antigen Cd8b proteinCD8B proteinCD8B1 protein |
Information sourced from Uniprot.org