| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.001 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | CD81 |
| Gene ID: | 975 |
| Uniprot ID: | CD81_HUMAN |
| Immunogen Region: | Phe113-Lys201 |
| Immunogen: | Recombinant Human CD81 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe113-Lys201 ) of Human CD81 (Accession #NP_004347.1) fused with hFc tag at the N-terminus. |
| Immunogen Sequence: | CNLSQTHSLNNRRTLMLMAQ MRRISPFSCLKDRHDFEFPQ EEFDGNQFQKAQAISVLHEM MQQTFNLFSTKNSSAAWDET LLEKFYIELFQQMNDLEACV IQEVGVEETPLMNEDSILAV KKYFQRITLYLMEKKYSPCA WEVVRAEIMRSLSFSTNLQK RLRRKD |
| Tissue Specificity | Expressed on B cells (at protein level). Expressed in hepatocytes (at protein level). Expressed in monocytes/macrophages (at protein level). Expressed on both naive and memory CD4-positive T cells (at protein level). |
| Post Translational Modifications | Not glycosylated. Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts. |
| Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction. In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption. May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels. Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms. |
| Protein Name | Cd81 Antigen 26 Kda Cell Surface Protein Tapa-1 Target Of The Antiproliferative Antibody 1 Tetraspanin-28 Tspan-28 Cd Antigen Cd81 |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-977606 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Associates With Cldn1 And The Cldn1-Cd81 Complex Localizes To The Basolateral Cell Membrane |
| Alternative Protein Names | Cd81 Antigen protein 26 Kda Cell Surface Protein Tapa-1 protein Target Of The Antiproliferative Antibody 1 protein Tetraspanin-28 protein Tspan-28 protein Cd Antigen Cd81 protein CD81 protein TAPA1 protein TSPAN28 protein |
Information sourced from Uniprot.org
