| Host: |
Rabbit/Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This CD5L antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Purification: |
Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Determination Method: |
Colormetric |
| Gene Symbol: |
CD5L |
| Gene ID: |
922 |
| Uniprot ID: |
CD5L_HUMAN |
| Immunogen: |
Capture immunogen: Recombinant Human CD5L protien.Detection Immunogen: Recombinant Human CD5L protien. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Not N-glycosylated. Probably not O-glycosylated. |
| Function | Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis. Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy. |
| Protein Name | Cd5 Antigen-LikeApoptosis Inhibitor Expressed By MacrophagesHaimCt-2Igm-Associated PeptideSp-Alpha |
| Cellular Localisation | SecretedCytoplasmSecreted By Macrophages And Circulates In The BloodTransported In The Cytoplasm Via Cd36-Mediated Endocytosis |
| Alternative Antibody Pair Names | Cd5 Antigen-Like antibody pairApoptosis Inhibitor Expressed By Macrophages antibody pairHaim antibody pairCt-2 antibody pairIgm-Associated Peptide antibody pairSp-Alpha antibody pairCD5L antibody pairAPI6 antibody pairUNQ203 antibody pairPRO229 antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
