| Host: | Mammalian Cells |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Active-Human CD50/ICAM3-C-hFc-His protein was developed from mammalian cells and has a target region of C-hFc-His. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity: | Measured by the ability of the Immobilised protein to support the adhesion of THP-1 cells. When cells are added to CD50/ICAM3 coated plates (12.5 Mu g/mL, 100 Mu L/well) , approximately >50% cells will adhere specifically after 60 minutes at 37 °C. |
| Gene Symbol: | ICAM3 |
| Gene ID: | 3385 |
| Uniprot ID: | ICAM3_HUMAN |
| Immunogen Region: | Gln30-His485 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated.glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. |
| Protein Name | Intercellular Adhesion Molecule 3Icam-3Cdw50Icam-RCd Antigen Cd50 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-5621575 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Intercellular Adhesion Molecule 3 proteinIcam-3 proteinCdw50 proteinIcam-R proteinCd Antigen Cd50 proteinICAM3 protein |
Information sourced from Uniprot.org
