• Biotinylated Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human CD45 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-CD45 Antibody at 2 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0. 16 Mu g/ml determined by ELISA.

Human CD45/PTPRC protein (Recombinant) (C-His-Avi) {Biotin} (STJP001724)

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STJP001724

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human CD45/PTPRC-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: PTPRC
Gene ID: 5788
Uniprot ID: PTPRC_HUMAN
Immunogen Region: Gln26­-Lys416
Immunogen: DNA sequence encoding Human CD45/PTPRC including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Heavily N- and O-glycosylated.
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase C
Leukocyte Common Antigen
L-Ca
T200
Cd Antigen Cd45
Database Links Reactome: R-HSA-202427
Reactome: R-HSA-416700
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative Protein Names Receptor-Type Tyrosine-Protein Phosphatase C protein
Leukocyte Common Antigen protein
L-Ca protein
T200 protein
Cd Antigen Cd45 protein
PTPRC protein
CD45 protein

Information sourced from Uniprot.org

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